Journal: PLoS ONE

Article Title: BMP4 Increases the Expression of TRPC and Basal [Ca 2+ ] i via the p38MAPK and ERK1/2 Pathways Independent of BMPRII in PASMCs

doi: 10.1371/journal.pone.0112695

Figure Lengend Snippet: Representative blots (A) and normalized bar graphs (B) of BMPRII, ActRIIa, ActRIIb, ALK2, BMPRIa and BMPRIb protein as detected by Western blotting in PASMCs treated with 50 ng/ml BMP4 for 60 h. Bar graph shows mean protein expression for BMPRII, ActRIIa, ActRIIb, ALK2, BMPRIa and BMPRIb protein relative to α-actin (n = 4 in each group; * P <0.05 vs. vehicle control). Bar values are as means ± SEM.

Article Snippet: All antibodies were obtained as follow: TRPC1, 6 antibodies (abcam), TRPC4 antibody (Alomone Laboratories), phospho-Smad1/5/8, phospho-ERK1/2, phospho-p38MAPK, ERK1/2, Smad5 and p38MAPK antibodies (Cell Signaling Technology), BMPRIa antibody (Proteintech Group), BMPRIb antibody (Invitrogen), ALK2 antibody (Novus), BMPRII antibody (BD), ActR2a and ActR2b antibodies (Abbiotec), α-actin antibody (Sigma-Aldrich).

Techniques: Western Blot, Expressing

Journal: Mediators of Inflammation

Article Title: Intestinal Mucosal Barrier Is Injured by BMP2/4 via Activation of NF- κ B Signals after Ischemic Reperfusion

doi: 10.1155/2014/901530

Figure Lengend Snippet: (a) and (b), (c) BMPRIa and BMPRII expression was detected by Western blotting and immunofluorescence staining. BMPRIa and BMPRII expression levels were both significantly increased after 6 h of hypoxia in IEC-6 cells. ** P < 0.01 versus control. BMPRIa and BMPRII expression in the intestinal mucosa also increased after I/R for 1 h compared to the control. Data are representative of 3 similar experiments.

Article Snippet: Anti-BMP2, anti-BMP4, anti-BMPRIa, anti-BMPRII, anti-BMPRIb, and anti-occludin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining